法医学杂志

法医学杂志 ›› 2003, Vol. 0 ›› Issue (2): 81-83.

• 论文 • 上一篇    下一篇

亚急性黄药子中毒的实验病理学研究

苏莉,朱建华,程利宝,李永宏   

  1. 皖南医学院法医学系,皖南医学院法医学系,皖南医学院法医学系,皖南医学院法医学系 安徽 芜湖 241001 ,安徽 芜湖 241001 ,安徽 芜湖 241001 ,安徽 芜湖 241001
  • 发布日期:2003-04-25 出版日期:2003-04-28

Experimental pathological study of subacute intoxication by Dioscorea bulbifera L

SU LI,ZHU JIAN-HUA,CHENG LI-BAO,ET AL. (DEPARTMENT OF FORENSIC PATHOLOGY,WANNAN MEDICAL COLLEGE,WUHU 241001,CHINA)   

  • Online:2003-04-25 Published:2003-04-28

被引次数

59

摘要： 目的 研究黄药子对小鼠毒性作用的病理变化及其毒作用机制。方法 按1/4LD_(50),1/10LD_(50),1/30LD_(50)剂量给ICR小鼠连续灌服200％的黄药子水煎剂,观察中毒小鼠各主要器官的组织学与超微结构变化,酶组织化学检查及血清生化检测。结果 中毒小鼠肝细胞脂肪变性,糖原增多,严重者可见灶性坏死;肾小管上皮细胞变性、坏死。通过酶组织化学方法发现肝脏SDH酶和G-6-P酶活性下降,血清ALT和BUN升高。结论 黄药子毒作用的主要靶器官或靶组织为肝和肾。毒作用机理是对线粒体、内质网等膜性结构的破坏导致酶活性的降低,影响代谢。

关键词: 黄药子, 小鼠, 中毒, 法医病理学

Abstract: Objective To study the pathological change and the toxic mechanism of Dioscorea bulbifera L in mice. Methods Sixty ICR mice were randomly assigned to four groups poisoned respectively with 200% Dioscorea bidbifera L of 1/4LD50,1/10LD50,1/30LD50 and a control group treated with distilled water by oral administration. All animals were pathologically examined with LM and some of them were examined with TEM when the mice died during the experiment or the survival mice were sacrificed after thirty days. Results The pathological changes showed fatty change and the increasing glycogen of liver cells;degeneration and necrosis of the epithelia of uriniferous tubules. The serum BUN and ALT of the experimental groups mice were higher than that of control group. Enzyme histochemical staining showed the decreasing activity of G-6-P and SDH in the liver cells in the experimental groups. Conclusion The experiment suggests that the target organs were liver and kidney. The toxic mechanism of Dioscorea bublifera L was the damage of the mitochondrional and endoplasmic reticulum membrane directly. As a result,the activity of the SDH and G-6-P decreased,the metabolism was affected.

Key words: Dioscorea bulbifera L, mice, subacute intoxication, forensic pathology