法医学杂志 ›› 2006, Vol. 0 ›› Issue (1): 7-9+97.

• 论文 • 上一篇    下一篇

DNA降解与腐败尸体死亡时间的相关性

罗光华;陈玉川;成建定;王江峰;高翠莲;   

  1. 广东司法警官职业学院司法鉴定系,广东警官学院,中山大学中山医学院法医病理教研室,中山大学中山医学院法医病理教研室,广东警官学院 广东广州510520,广东广州510232,广东广州510089,广东广州510089,广东广州510232
  • 发布日期:2006-02-25 出版日期:2006-02-28

Relationship between DNA Degradation and Postmortem Interval of Corrupt Corpse

LUO GUANG-HUA1, CHEN YU-CHUAN2, CHENG JIAN-DING3, WANG JANG-FENG3, GAO CUI-LIAN2 (1.GUANGDONG JUSTICE POLICE VOCATIONAL COLLEGE, GUANGZHOU 510520, CHINA; 2.GUANGDONG POLICE COLLEGE, GUANGZHOU 510232,CHINA; 3.DEPARTMENT OF FORENSIC PATHOLOGY, ZHONGSHAN MED   

  • Online:2006-02-25 Published:2006-02-28

摘要: 目的探讨骨髓细胞核DNA含量变化与腐败尸体死亡时间的关系。方法应用DNA高度特异性染色方法Feulgen改良法结合计算机图像分析技术对死后不同时间(0~14d)胸骨骨髓细胞核DNA含量变化进行分析。结果胸骨骨髓细胞核DNA含量随死亡时间延长呈缓慢而规律下降,直至死后两周仍未完全降解。结论死后DNA降解速率与死亡时间呈线性关系,测定死后骨髓细胞核DNA含量可望成为腐败尸体死亡时间推断的新方法。

关键词: 死亡时间推断, DNA含量, 腐败尸体, 胸骨骨髓

Abstract: Objective To study the relationship between DNA degradation and postmortem interval of corrupt corpse. Methods By determining the marrow DNA content with histochemical technique and image analysis. Results The content of marrow DNA decreased gradually with prolongation of postmortem interval, and it evencould be detected till 14 days after death. Conclusion There was a linear relationship between the degradation rate of the nuclear DNA and postmortem interval.

Key words: corrupt corpse, DNA degradation, marrow of breast bone, postmortem interval