法医学杂志

法医学杂志 ›› 2012, Vol. 28 ›› Issue (3): 198-200.DOI: 10.3969/j.issn.1004-5619.2012.03.010

• 论著 • 上一篇    下一篇

河豚毒素在死后豚鼠组织和体液中的分布

刘  伟，达  情，沈  敏   

  1. （司法部司法鉴定科学技术研究所 上海市法医学重点实验室，上海 200063）
  • 发布日期:2012-06-25 出版日期:2012-06-28
  • 作者简介:刘伟（1963—），女，江苏徐州人，主任法医师，硕士研究生导师，主要从事法医毒物学研究；E-mail：liuw@ssfjd.cn
  • 基金资助:

    上海市科委社会发展领域重点科技项目（072512019）；中央级公益性科研院所基本科研业务费专项（GY0702）

Postmortem Distribution of Tetrodotoxin in Tissues and Body Fluids of Guinea Pigs

LIU WEI, DA QING, SHEN MIN   

  1. （Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, P.R.China,Shanghai 200063, China）
  • Online:2012-06-25 Published:2012-06-28

被引次数

2

摘要： 目的 研究河豚毒素在死后豚鼠组织和体液中的分布规律，为河豚毒素中毒的法医学鉴定和临床诊治提供方法和评价依据。 方法 建立河豚毒素的豚鼠灌胃染毒致死模型，豚鼠分别以100、50、15 μg/kg 3种剂量河豚毒素灌胃后，观察其中毒症状，待豚鼠死亡后立即解剖，分别取心、肝、脾、肺、肾、脑、胃、肠、胆汁、心血和尿液，用LC-MS/MS检测其中河豚毒素含量。 结果 豚鼠河豚毒素灌胃染毒后，均出现呼吸急促、精神萎靡等中毒症状继而死亡。肺、胃、肠、尿液中河豚毒素含量较高，其次为血液、心、脑，胆汁中含量最低。 结论 河豚毒素在豚鼠体内死后分布不均匀，肺、胃、肠、尿液和血液中含量较高，可作为疑似河豚中毒检测河豚毒素的较佳检材。

关键词: 法医毒理学, 河豚毒素, 液相色谱-串联质谱法, 死后分布, 豚鼠

Abstract: Objective To investigate the postmortem distribution of tetrodotoxin in tissues and body fluids of guinea pig, and to provide method and evidence for forensic identification and clinical diagnosis and treatment. Methods Guinea pigs were intragastric administrated with 100, 50, 15 μg/kg tetrodotoxin, respectively. The poisoning symptoms were observed. The samples of heart, liver, spleen, lung, kidney, brain, stomach, intestines, bile, heart blood and urine were collected. The concentrations of tetrodotoxin in tissues and body fluids were measured with liquid chromatography-tandem mass spectrometry（LC-MS/MS）. Results After administrated with tetrodotoxin, all guinea pigs came out poisoning signs including tachypnea, weary and dead finally. Tetrodotoxin concentrations in lung, stomach, intestines and urine were higher, followed by blood, heart and brain. The concentration in bile was the lowest. Conclusion Postmortem distribution of tetrodotoxin in guinea pig is uneven. The concentration in the lung, stomach, intestines, urine and heart blood are higher, those tissues could be used for diagnosis of tetrodotoxin poisoning.

Key words: forensic toxicology, tetrodotoxin, LC-MS/MS, postmortem distribution, guinea pigs

中图分类号: