Progress in the 16S rRNA Gene Sequencing in Forensic Science
SONG Guo-qing1,2,3, CAO Yu2,3, LI Hui2,3, MA Ke2,3, ZhAO Xue-ying2,3, ZOU Kai-nan2,3, ZHOU Huai-gu1,2,3
1. Department of Forensic Medicine, School of Basic Medical Science, Fudan University, Shanghai 200032, China; 2. Shanghai Key Laboratory of Crime Scene Evidence, Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China; 3. Shanghai Research Institute of Criminal Science and Technology, Shanghai 200083, China
Forensic microorganism is one of the hotspots of forensic science research. Due to its conservatism and specificity, the 16S rRNA gene is found to be an ideal marker for forensic identification. With the rapid development of high throughput sequencing technology, the research on microorganisms has been gradually applied to many fields such as environment and health care. In the field of forensic science, the results of forensic microbiology research, represented by 16S rRNA gene sequencing, are also gradually applied to forensic practice, such as biological samples identification, individual identification, postmortem interval estimation, and regional inference, which not only provide clues for the investigation of cases but also complement and assist traditional methods. This paper describes the research methods and related sequencing technologies of 16S rRNA gene sequencing, summarizes its research progress, and discusses the application value and potential of 16S rRNA in forensic science.