法医学杂志

法医学杂志 ›› 2019, Vol. 35 ›› Issue (6): 677-681.DOI: 10.12116/j.issn.1004-5619.2019.06.006

• 论著 • 上一篇    下一篇

全血中4种新型合成大麻素的快速检测

徐恩宇1， 张云峰2， 宋歌2， 蒋锐1， 刘光琴1， 刘俊亭1   

  1. 1. 中国医科大学法医毒物分析教研室，辽宁 沈阳 110122； 2. 公安部物证鉴定中心，北京 100038
  • 发布日期:2019-12-25 出版日期:2019-12-28
  • 通讯作者: 张云峰，女，副研究员，主要从事法医毒理学研究；E-mail：yunfeng_2002@sohu.com
  • 作者简介:徐恩宇（1987—），男，讲师，公安部物证鉴定中心访问学者，主要从事毒物分析研究；E-mail：xey626@163.com
  • 基金资助:
    国家自然科学基金面上资助项目（81671864）；国家重点研发计划资助项目（2017YFC0803504）；中国医科大学校内基金资助项目（XZR20160033）；中国医科大学大学生创新训练资助项目（201710159000053，201710159000191）

Rapid Identification of Four New Synthetic Cannabinoids in Whole Blood

XU En-yu1, ZHANG Yun-feng2, SONG Ge2, JIANG Rui1, LIU Guang-qin1, LIU Jun-ting1   

  1. 1. Department of Forensic Analytical Toxicology, China Medical University, Shenyang 110122, China; 2. Institute of Forensic Science, Ministry of Public Security, PRC, Beijing 100038, China
  • Online:2019-12-25 Published:2019-12-28

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摘要： 目的 建立准确、快速的方法鉴定血液中4种新型合成大麻素（JWH-203、JWH-122、5F-APINACA和AB-CHMINACA）。 方法 全血样品经乙腈-甲醇提取后，经气相色谱-质谱（gas chromatography-mass spectrometry，GC-MS）进行筛选，采用液相色谱-串联质谱（liquid chromatography-tandem mass spectrometry，LC-MS/MS）进行确认，采用多反应监测模式进行定量分析。 结果 GC-MS法于21 min完成分析，LC-MS/MS法于5 min完成分析，AB-CHMINACA、JWH-203、5F-APINACA和JWH-122均采用准分子离子峰作为母离子，定量离子对分别为m/z 357.4→312.2、m/z 340.2→125.0、m/z 384.1→135.1、m/z 356.4→169.2。血液中4种合成大麻素在1~250 ng/mL质量浓度范围内线性良好（r>0.99），检出限为0.1~0.5 ng/mL，提取回收率为85.4%~95.2%，精密度小于10.0%，基质效应为80.3%~92.8%。 结论 本研究得到了4种合成大麻素的GC-MS和LC-MS/MS色谱行为和质谱解析信息，初步讨论了色谱行为差异的可能原因，对判断合成大麻素的发展趋势有提示作用。本方法适用于血液中4种合成大麻素的快速检测，在目前新型合成大麻素滥用激增的情况下，可为此类物质的鉴定提供参考。

关键词: 法医毒理学, 气相色谱-质谱法, 液相色谱-串联质谱法, 合成大麻素类, 血液

Abstract: Objective To establish accurate and rapid methods to identify four new synthetic cannabinoids （JWH-203, JWH-122, 5F-APINACA and AB-CHMINACA） in blood samples. Methods The whole blood samples were extracted by acetonitrile and methanol, screened by gas chromatography-mass spectrometry （GC-MS） then confirmed by liquid chromatography-tandem mass spectrometry （LC-MS/MS） method, and multiple reaction monitoring （MRM） mode was used for quantitative analysis. Results The GC-MS method needed 21 min to complete the analysis, while the LC-MS/MS method needed 5 min. The AB-CHMINACA, JWH-203, 5F-APINACA and JWH-122 all used quasi molecular ion peak as a parent ion. The precursor-product ion combinations were m/z 357.4→312.2, m/z 340.2→125.0, m/z 384.1→135.1 and m/z 356.4→169.2. The four synthetic cannabinoids in blood samples had good linearity in the 1-250 ng/mL mass concentration range （r>0.99）. The limits of detection （LODs） were in the range of 0.1-0.5 ng/mL, the recovery rate was 85.4%-95.2%, the RSD less than 10.0%, and the matrix effect was 80.3%-92.8%. Conclusion The GC-MS and LC-MS/MS chromatographic behaviors and mass spectrometry analysis information of four synthetic cannabinoids were obtained in this study, and the possible causes of differences in chromatographic behaviors were discussed preliminarily. Therefore this study has a suggestive effect on judging the development trend of synthetic cannabinoids. This method can be used for rapid identification of four synthetic cannabinoids in blood, which can provide reference for identification of new synthetic cannabinoids when they are proliferating at present.

Key words: forensic toxicology, gas chromatography-mass spectrometry, liquid chromatography-tandem mass spectrometry, synthetic cannabinoids, blood