Abstract: Objective To establish standardized methods and parameters of the isolated heart coronary angiography through the experiment of in vitro porcine heart by MSCT. Methods Based on different perfusion volume （50, 60 and 70 mL） and different perfusion-imaging time （5, 10 and 20 min）, the in vitro porcine coronary artery was injected liposoluble and water-soluble contrast agents using remodel angiography equipment and scanned by MSCT. And the 3D image results were compared. The images were recorded and evaluated by 2 radiologists and analyzed by statistical software. Results Liposoluble contrast agent affected the images by damaging and infiltrating the fats around the coronary artery, while the water-soluble contrast agent didn’t affect the images. The groups with 60 mL or 70 mL perfusion and 5 min perfusion-imaging time had the best images. Conclusion The suitable parameters of the angiography lay the foundation of postmortem coronary angiography.

Key words: forensic pathology, angiography, virtopsy, contrast agents, coronary