Abstract: Objective The study was carried out on application of CODIS loci (FGA, vWA, CSF1PO, TH01,TPOX, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51 and D21S11) in 100 cases of excluding paternity. Methods The PCR amplified products of Profiler Plus and Cofiler amplification kit were injected into a capillary on the ABI PRISM 310 Genetic Analyzer. GeneScan software analyzed the collected data, which can then be imported into Genotyper software for genotyping of alleles.Results In the group of Mother－ Child－ Alleged Father, more than 3 STR loci incompatibilities between alleged father and child were found in all observed cases, the mean incompatibility was 6.63 ; In the group of Alleged Father－ Child, 94.0％ of all cases observed was found more than 3 STR loci incompatibilities, the mean incompatibility was 5.01.Conclusion The results showed that CODIS loci had good application to excluding paternity, and the choice of hyperpolymorphic markers in investigation, which were valued by DP, H and PE, had direct relation with the augmentation of incompatibilities in excluding paternity. [

Key words: CODIS loci, STR, paternity investigation, excluding paternity