Simultaneous quantification of cannabinoids and the major metabolite,THC-COOH in human hair

Abstract

Abstract: Objective To propose a method for the simultaneous identification and quantification of THC,CBN and THC-COOH in human hair.Methods The hair samples were decontaminated,added chlofenamic acid as the internal standard,hydrolyzed with sodium hydroxide,extracted with n-hexane ∶ ethyl acetate(9:1),derivatized,and analyzed by GC/MS-SIM.Results The limits of detection for THC,CBN and THC-COOH were0.01,0.05and0.01ng/mg,respectively.THC was detected in the10hair samples with the range from0.11to8.84ng/mg.THC-COOH was not detected in three samples out of ten.The concentrations of rest seven samples were too low to be quantified.Conclusion The concentrations of THC-COOH were lower than that of THC in hair obviously.

Key words: cannabinoids, THC-COOH, hair, GC/MS

XIANG PING;SHEN MIN;SHEN BAO-HUA;ET AL.( INSTITUTE OF FORENSIC SCIENCE;MINISTRY OF JUSTICE;PRC;SHANGHAI000 ). Simultaneous quantification of cannabinoids and the major metabolite,THC-COOH in human hair[J]. , 2002, 18(4): 216-219.

[1]	Jiao-jiao JI, Xin WANG, Jia-man LIN, Duo-qi XU, Hui YAN, Min SHEN. Research Progress on the Application of MALDI-MSI in Hair Analysis [J]. Journal of Forensic Medicine, 2024, 40(6): 542-549.
[2]	Ming-hui JIN, Ya LI, Shao-hua YI. Research Progress on the Application of Human Body Surface Microbiome to Forensic Individual Identification [J]. Journal of Forensic Medicine, 2024, 40(4): 379-386.
[3]	Cui-mei LIU, Wei JIA, Chun-hui SONG, Zhen-hua QIAN, Zhen-dong HUA, Yue-meng CHEN. Qualitative and Quantitative Analysis of Five Indoles or Indazole Amide Synthetic Cannabinoids in Suspected E-Cigarette Oil by GC-MS [J]. Journal of Forensic Medicine, 2023, 39(5): 457-464.
[4]	Hang CHEN, Jing HU, Zheng QIAO, Hong-xiao DENG, Min LÜ, Wei LIU. Research Progress on Biological Matrix Reference Materials in Forensic Toxicology [J]. Journal of Forensic Medicine, 2023, 39(2): 176-185.
[5]	Jiao-jiao JI, Duo-qi XU, Ping XIANG, Hui YAN, Min SHEN. Analysis of Forty-Two Psychoactive Substances in a Single Hair by Micro-Segmental Technique [J]. Journal of Forensic Medicine, 2023, 39(2): 151-160.
[6]	SHI Yan, ZHOU Li-ying, SHEN Bao-hua, et al.. Analysis and Application of Seven Common New Psychoactive Substances of Synthetic Cannabinoids in Hair [J]. Journal of Forensic Medicine, 2021, 37(4): 479-485，492.
[7]	MENG Xin, HUA Zhen-dong, JIA Wei, at al.. Identification of Synthetic Cannabinoid 5F-EDMB-PICA in Suspicious Herbal Products [J]. Journal of Forensic Medicine, 2021, 37(4): 486-492.
[8]	YUE Lin-na, XIANG Ping, SONG Fen-yun, et al.. Metabolism of New Psychoactive Substances 4F-MDMB-BUTINACA in Zebrafish [J]. Journal of Forensic Medicine, 2021, 37(4): 493-499.
[9]	CHENG Feng, ZHANG Qing-xia, CHEN Cheng-jian, et al.. Mitochondrial DNA Heteroplasmy of Hair Shaft Using HID Ion GeneStudioTM S5 Sequencing System [J]. Journal of Forensic Medicine, 2021, 37(1): 21-25.
[10]	YAN Hui, SHI Ge-fei, SHEN Min. Bibliometrics Analysis of Forensic Toxicology Literature of Mainland Chinese Scholars in SCIE Journals#br# [J]. 法医学杂志, 2019, 35(6): 667-676.
[11]	XU En-yu, ZHANG Yun-feng, SONG Ge,et al.. Rapid Identification of Four New Synthetic Cannabinoids in Whole Blood [J]. 法医学杂志, 2019, 35(6): 677-681.
[12]	MENG Hao-tian, LAN Qiong, ZHU Bo-feng. DNA Molecular Identification of Human Phenotypic Characteristics——New Progress Over the Past Five Years [J]. 法医学杂志, 2019, 35(5): 512-518，524.
[13]	REN Guan-heng, YAN Qi-jiang, TANG Ying,et al.. Research Progress on Detection Methods of Amphetamines in Human Hair [J]. 法医学杂志, 2019, 35(1): 89-92.
[14]	PAN Mei-ru, QIANG Huo-sheng, SHEN Bao-hua,et al.. Rapid Determination of Cocaine and Its Metabolite Benzoylecgonine in Hair by LC-MS/MS [J]. 法医学杂志, 2018, 34(4): 375-378，383.
[15]	LI FU, ZHANG-MIN, WANG YING-XI, DENG , ET AL.. Determination of Hair Shafts by InnoTyper?誖21 Kit [J]. , 2017, 33(6): 615-618.

Simultaneous quantification of cannabinoids and the major metabolite,THC-COOH in human hair

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