A study of genetic polymorphism of the STR locus D7S2846、D19S400 and D18S535 in Han popula-tion in Wenzhou

Abstract

Abstract: Objective To study genetic polymorphism of D7S2846、D19S400and D18S535in Han population in Wenzhou.Methods DNA was extracted with chelex-100method from EDTA-blood samples of194unrelated individuals in Wenzhou and amplified with PCR technique.The PCR products were analyzed by PAG vertical electrophoresis and silver-stain.Results6alleles and15genotypes of D7S2846、10alleles and36genotypes of D19S400、8alleles and26genotypes of D18S535was observed.The heterozygosities of the three loci are0.644,0.724and0.772;The power of discriminating are0.854,0.940and0.938.Conclusion The heterozygosities of the three loci are high and the frequencies was in accordance with Hardy-Weinberg equilibrium(P>0.05),so the three loci can be used in forensic medicine and in other genetic researches.

Key words: short tandem repeats, polymerase chain reaction, genetic polymorphism

ZHANG HONG-QIN;WU SHU-ZHEN;BI YUN-TIAN(MOLECULAR BIOLOGY RESEARCH CENTRE OF WENZHOU MEDICAL COL-LEGE;WENZHOU0;CHINA). A study of genetic polymorphism of the STR locus D7S2846、D19S400 and D18S535 in Han popula-tion in Wenzhou[J]. , 2004, 0(3): 160-161.

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A study of genetic polymorphism of the STR locus D7S2846、D19S400 and D18S535 in Han popula-tion in Wenzhou

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