Changes of RyR_2 mRNA Expression in Rats Suffering from Acute Myocardial Ischemia

Abstract

Abstract: Objective To investigate the changes of ryanodine receptor 2(RyR2) mRNA expression in rats suffering from acute myocardial ischemia. Methods SD rats were divided randomly into normal control group,myocardial ischemia group and sudden death group. The models of myocardial ischemia and sudden cardiac death were induced by intraperitoneal injection of hypophysine. The changes of RyR2 mRNA expression in cardiac sarcoplasmic reticulum(SR) of rats suffering from myocardial ischemia were detected by fluorescent RT-PCR technique. Results The levels of RyR2 mRNA in the myocardial ischemia group and sudden death group were significant lower than those in the control group(P<0.05). Conclusion Myocardial ischemia may induce down-regulation of cardiac SR RyR2 mRNA expression.

Key words: forensic pathology, myocardial ischemia, ryanodine receptor calcium release channel, reverse transcriptase polymerase chain reaction

JIE YA;ZHU SHAO-HUA;JIANG YAN-WEI;ZHANG LEI;FAN FAN (. DEPARTMENT OF FORENSIC MEDICINE;TONGJI MEDICAL COLLEGE;HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY;WUHAN 000;CHINA;. TIANJIN INSTITUTE OF CRIMINAL SCIENCE AND TECHNOLOGY;TIANJIN 00;CH. Changes of RyR_2 mRNA Expression in Rats Suffering from Acute Myocardial Ischemia[J]. , 2008, 0(5): 327-329+.

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Changes of RyR_2 mRNA Expression in Rats Suffering from Acute Myocardial Ischemia

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