Abstract: Objective To obtain data of polymorphism distribution of 10 short tandem repeat (STR) loci D1S2145, D3S2433, D5S1507, D5S2502, D8S2319, D9S926, D16S767, D17S2181, GATA140E03, GA- TA196B10 in Chinese Han population in Chengdu and to evaluate their usefulness in the field of forensic science and their species specificity. Methods DNA of 100 unrelated individuals of Chengdu Han population was extracted with Chelex method, amplified by PCR, then typed with silver staining after polyacry- lamide gel electropho-resis(PAGE). Ten different animals were selected as the controls in this study for evaluating the species specificity of the ten STR loci. Results In the ten STR loci of Chengdu Han population, 6, 5, 8, 5, 6, 7, 7, 5, 7 and 7 alleles were found, respectively. 17, 14, 28, 15, 16, 18, 15, 14, 19 and 21 genotypes were observed in the ten loci, respectively. The allele and genotype frequency distributions of the ten loci were detected no deviation from the Hardy-Weinberg law of equilibrium. By comparison with the data from 10 different animals, the species specificity of D3S2433, D5S1507, D5S2502, D8S2319 and GATA196B10 was good, but part of animals had amplification product at typing field of the other loci. Conclusion The 10 STR loci mentioned above are highly polymorphic and can be used in the forensic personal identification and paternity testing.

Key words: forensic genetics, polymorphism, genetic, species specificity, short tandem repeat, Chengdu, Han nationality