Abstract: Objective To explore the expression of tryptase and chymase in human lung tissue of anaphylactic shock and its value for forensic medicine.Methods With ten carbon monoxide poisoning cases as control group,the levels of tryptase and chymase were observed by immunofluorescence and analyzed using the Image Analyze and the Image-pro plus 5.0.2.The positive mast cells were counted and the levels of the tryptase and chymase were calculated respectively.Results There was a statistically significant difference(P<0.05) for the tryptase and chymase concentrations in the lung tissue between the anaphylactic shock group and the control group.Conclusion The levels of the tryptase and the chymase expression are greatly increased in human lung tissue of anaphylactic shock,which may provide the morphological evidence and reference for the diagnosis of anaphylactic shock in forensic practice.

Key words: forensic pathology, anaphylaxis, lung, tryptases, chymases