Simultaneous quantification of cannabinoids and the major metabolite,THC-COOH in human hair

Abstract

Abstract: Objective To propose a method for the simultaneous identification and quantification of THC,CBN and THC-COOH in human hair.Methods The hair samples were decontaminated,added chlofenamic acid as the internal standard,hydrolyzed with sodium hydroxide,extracted with n-hexane ∶ ethyl acetate(9:1),derivatized,and analyzed by GC/MS-SIM.Results The limits of detection for THC,CBN and THC-COOH were0.01,0.05and0.01ng/mg,respectively.THC was detected in the10hair samples with the range from0.11to8.84ng/mg.THC-COOH was not detected in three samples out of ten.The concentrations of rest seven samples were too low to be quantified.Conclusion The concentrations of THC-COOH were lower than that of THC in hair obviously.

Key words: cannabinoids, THC-COOH, hair, GC/MS

XIANG PING;SHEN MIN;SHEN BAO-HUA;ET AL.( INSTITUTE OF FORENSIC SCIENCE;MINISTRY OF JUSTICE;PRC;SHANGHAI000 ). Simultaneous quantification of cannabinoids and the major metabolite,THC-COOH in human hair[J]. , 2002, 0(4): 216-219.

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Simultaneous quantification of cannabinoids and the major metabolite,THC-COOH in human hair

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