Abstract: Objective To study the relation between human blood estazolam concentration and neurobehavioral function.Methods The neurobehavioral ability of 10 volunteers were measured with computer-administered neurobehavioral evaluation system-chinese3(NES-C3) and SMART EquiTest system.Results The neurobe-havioral ability and balance function declined 1 h later after dosing estazolam.The neurobehavioral ability index and balance function declined to the lowest level 3 h later after dosing estazolam.The neurobehavioral ability recovered partly 6 h later after dosing estazolam,and neurobehavioral ability recovered completely 10 h later.Conclusion Driving ability was impaired when estazolam concentration in blood is 20 ng/mL,and the neurobehavioral ability declined when estazolam concentration is 40 ng/mL in blood.The influence to hu-man in absorption process is greater than the metabolic process with the same estazolam concentration.

Key words: forensic toxicology, estazolam, neurobehavioral manifestations