法医学杂志

法医学杂志 ›› 2019, Vol. 35 ›› Issue (4): 444-447.DOI: 10.12116/j.issn.1004-5619.2019.04.013

• 技术与应用 • 上一篇    下一篇

运用18S rDNA克隆文库检测滇池硅藻种群多样性

胡蝶1， 皮之云2， 张志荣3， 陈燕祥4， 邢豫明2，4， 程宝文2，4   

  1. 1. 中国人民解放军陆军军医大学，重庆 400038； 2. 昆明医科大学，云南 昆明 650500； 3. 中国科学院昆明植物研究所，云南 昆明 650204； 4. 云南省公安厅，云南 昆明 650228
  • 发布日期:2019-08-25 出版日期:2019-08-28
  • 通讯作者: 程宝文，男，博士，主任法医师，硕士研究生导师，主要从事法医物证学、群体遗传学研究；E-mail：ggcbw@vip.sina.com
  • 作者简介:胡蝶（1987—），女，硕士研究生，主要从事法医病理学研究；E-mail：532726a00qg.cdb@sina.cn

Application of 18S rDNA Clone Library to Detect Diatom Population Diversity in Dianchi#br#

HU Die1, PI Zhi-yun2, ZHANG Zhi-rong3, CHEN Yan-xiang4, XING Yu-ming2,4, CHENG Bao-wen2,4   

  1. 1. Army Medical University, Chongqing 400038, China; 2. Kunming Medical University, Kunming 650500, China; 3. Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China; 4. Yunnan Provincial Public Security Department, Kunming 650228, China
  • Online:2019-08-25 Published:2019-08-28

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摘要： 目的 构建18S rDNA克隆文库检测滇池硅藻种群的多样性。 方法 用硅藻18S rDNA特异性引物对滇池6个采集点水样的硅藻进行DNA扩增，构建硅藻18S rDNA克隆文库，随机挑取克隆子进行测序，通过BLAST进行序列比对，分析滇池硅藻种群分布，运用MEGA v7.0.14软件构建滇池水域硅藻18S rDNA系统发育树。 结果 240个克隆子测序获得167条硅藻序列，包括冠盘藻属、等片藻属、直链藻属等11个硅藻种属，6个采集点间硅藻种群分布存在一定差异。 结论 滇池硅藻种群分布具有一定特征，硅藻18S rDNA序列分析对法医学溺死地点推断有一定参考价值。

关键词: 法医病理学, 法医遗传学, 硅藻, 溺死, 18S rDNA, 滇池

Abstract: Objective To detect the diatom population diversity in Dianchi by constructing a 18S rDNA clone library. Methods DNA from diatoms in 6 water samples of Dianchi was amplified with diatom 18S rDNA specific primer.The 18S rDNA clone library was constructed, and clones were randomly selected for sequence. Sequence alignment was performed by BLAST. The diatom population distribution in Dianchi was analyzed and the phylogenetic tree of diatom 18S rDNA in Dianchi waters was established with the MEGA v7.0.14 software. Results Two hundred and forty clones were sequenced, with 167 diatom sequences obtained, including 11 diatom species such as Stephanodiscus, Diatoma, and Melosira. There were certain differences in diatom population distribution among the 6 samples. Conclusion The population distribution of diatom species in Dianchi shows unique features and the sequence analysis of diatom 18S rDNA has a certain reference value to the inference of forensic drowning sites.

Key words: forensic pathology, forensic genetics, diatom, drowning, 18S rDNA, Dianchi