法医学杂志

法医学杂志 ›› 2012, Vol. 28 ›› Issue (2): 115-119.DOI: 10.3969/j.issn.1004-5619.2012.02.010

• 论著 • 上一篇    下一篇

氯胺酮与酒精对小鼠学习记忆行为的影响

杨美玉1，3，丁  飞2，蒋小岗3，吴勰星4，顾振纶3，郭次仪3，卞士中1   

  1. （1. 苏州大学医学部法医学系 苏州大学司法鉴定所，江苏 苏州 215123； 2. 上海市公安局宝山分局刑事科学研究所，上海 宝山 201900； 3. 苏州中药研究所，江苏 苏州 215006； 4. 苏州大学医学部临床医学系，江苏 苏州 215123）
  • 发布日期:2012-04-25 出版日期:2012-04-28
  • 通讯作者: 通信作者：卞士中，男，主任法医师，副教授，硕士研究生导师，主要从事法医毒理学研究；E-mail：bianshizhong@suda.edu.cn
  • 作者简介:杨美玉（1984—），女，山东济宁人，硕士研究生，主要从事法医毒理学研究；E-mail：meiyu0307@163.com

Effects of Ketamine and Alcohol on Learning and Memory Impairment in Mice

YANG MEI-YU1,3, DING FEI2, JIANG XIAO-GANG3, WU XIE-XING4, GU ZHEN-LUN3, GUO CI-YI3, BIAN SHI-ZHONG1   

  1. （1. Institute of Forensic Science of Soochow University, Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, China; 2. Institute of Criminal Science and Technology, Baoshan Branch of Shanghai Public Security Bureau, Shanghai 201900, China; 3. Suzhou Institute of Chinese Materia Medical, Suzhou 215006, China; 4. Department of Clinical Medicine, Medical College of Soochow University, Suzhou 215123, China）
  • Online:2012-04-25 Published:2012-04-28

摘要： 目的 研究氯胺酮与酒精对小鼠学习记忆的行为学及相关神经递质的影响。 方法 40只小鼠分为4组：对照组、氯胺酮组、酒精组、氯胺酮酒精联合给药组，每组10只。氯胺酮和酒精分别采用腹腔注射和灌胃给药的方式，1次/d，连续14 d。通过跳台和水迷宫实验检测各组小鼠的学习记忆能力，通过测定小鼠大脑组织中乙酰胆碱（acetylcholine，ACh）和5-羟色胺（5-hydroxy tryptamine，5-HT）的含量初步探讨氯胺酮与酒精对小鼠学习记忆的影响机制。 结果 （1）跳台实验：给药组小鼠均出现潜伏期缩短，错误次数增多（P＜0.05）。与单独给药相比，联合给药组错误次数显著增加。（2）水迷宫实验：给药组小鼠潜伏期显著增加（P＜0.05），平台所在象限活动时间明显缩短（P＜0.05），穿台次数显著减少（P＜0.05）。（3）生化指标测定：各给药组ACh和5-HT含量明显下降，且联合给药组较单独给药组下降更为明显（P＜0.05）。 结论 氯胺酮和酒精对小鼠学习记忆行为的抑制具有协同作用，这可能与共同抑制5-HT和ACh含量的降低有关。

关键词: 法医毒理学, 氯胺酮, 酒精, 学习, 记忆, 小鼠

Abstract: Objective To study the effects of ketamine and alcohol on learning and memory in mice and its possible mechanism. Methods Forty mice were divided into 4 groups： normal control group, ketamine group, alcohol group, and alcohol plus ketamine group. Ketamine and alcohol were given by intraperitoneal injection and intragastric administration, respectively, 1 time per day, for 14 days. The ability of learning and memory in mice was tested by the method of step-down and Morris water maze. Acetylcholine（ACh） and 5-hydroxy tryptamine（5-HT） in mice brain tissue were analyzed for the possible mechanism. Results （1） Step-down： The treatment groups lessened the latency and added wrong times（P＜0.05）. The number of errors in the combined treatment group significantly increased comparing with the single drug treatment group（P＜0.05）. （2） Morris water-maze： The treatment groups prolonged the latency（P＜0.05）, reduced the target quadrant activity time significantly（P＜0.05）, and decreased the numbers of crossing the former platform significantly（P＜0.05）. （3） Biochemical index determination： The concentrations of ACh and 5-HT in treatment groups decreased significantly（P＜0.05）, showed a more decreasement comparing with the single drug treatment group. Conclusion Ketamine has a synergistic effect with alcohol on learning and memory impairment in mice, which may be related to the common inhibitive effect on the ACh and 5-HT.

Key words: forensic toxicology, ketamine, alcohol, learning, memory, mice

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