法医学杂志

法医学杂志 ›› 2005, Vol. 0 ›› Issue (3): 174-176.

• 论文 • 上一篇    下一篇

肋软骨及牙髓细胞DNA含量与PMI的相关性

龙仁,王伟平,熊平,黄安,刘承泉   

  1. 南华大学法医病理学教研室,衡阳市中级人民法院研究室 ,南华大学法医病理学教研室,湖南省高级人民法院,湘潭市中级人民法院 湖南衡阳421001 ,湖南衡阳421001 ,湖南长沙410005 ,湖南湘潭411100
  • 发布日期:2005-06-25 出版日期:2005-06-28

Correlation between PMI and DNA Degradation of Costicartilage and Dental Pulp Cells in HumanBeing

LONG REN1,WANG WEI-PING2, XIONG PING1, ET AL. (1.DEPARTMENT OF FORENSIC MEDICINE, NANHUA UNIVERSI-TY, HENGYANG 421001,CHINA; 2.PEOPLE′S COURT OF HENGYANG, HENGYANG 421001,CHINA)   

  • Online:2005-06-25 Published:2005-06-28

被引次数

33

摘要： 目的探讨人死后肋软骨及牙髓细胞平均DNA含量与死亡时间(PMI)的相关性,寻求推断PMI的新方法。方法应用细胞图像分析技术分析高温(30～35℃)及低温(15～20℃)环境下人死后0～15d两种组织细胞平均DNA含量的降解情况。结果两种组织细胞平均DNA含量随死后时间的延长而逐步降解,其中低温组牙髓细胞平均DNA含量的降解在死后0～4d内有一个降解平台期。统计分析两组温度下两种组织细胞平均DNA含量与PMI有显著的负相关性(P<0.01)。结论依据两种组织细胞平均DNA含量的降解可进行PMI推断。

关键词: 死亡时间, 牙髓, 肋软骨, DNA降解

Abstract: Objective To probe the correlation between the postmortem interval (PMI) and the DNA degradationof costicartilage and dental pulp cells in human being after death ,and to seek a new method for estimating PMI.Methods The image cytometry was used to measure the DNA degradation under different ambient temperatures(30- 35℃,15- 20℃) in 0- 15 days after death. Results The average DNA content of two kinds of tissue wasdegradated with the prolongation of PMI. But there was a plateau period of 0～4 days for dental pulp cells ofhuman being in 15～20℃.There was a high negative correlativity P<0.01 between the average DNA content andPMI. Conclusion PMI could be estimated accurately according to the DNA degradation of costicartilage anddental pulp cells in human being after death.

Key words: postmortem interval, dental pulp, costicartilage, DNA degradation