法医学杂志

法医学杂志 ›› 2009, Vol. 0 ›› Issue (6): 431-433.

• 论文 • 上一篇    下一篇

气相色谱-串联质谱法测定血液中乙基葡萄糖醛酸苷

沈保华;严慧;赵晖;卓先义;   

  1. 司法部司法鉴定科学技术研究所上海市法医学重点实验室;复旦大学上海医学院;
  • 发布日期:2009-12-25 出版日期:2009-12-28

Determination of Ethyl Glucuronide in Blood by GC-MS/MS

SHEN BAO-HUA1, YAN HUI1, ZHAO HUI1,2, ZHUO XIAN-YI1 (1. SHANGHAI KEY LABORATORY OF FORENSIC MEDICINE, INSTITUTE OF FORENSIC SCIENCE, MINISTRY OF JUSTICE, P.R.CHINA, SHANGHAI 200063, CHINA; 2. SHANGHAI MEDICAL COLLEGE, FUDAN UNIVERSITY, SHANGHAI 200032, CH   

  • Online:2009-12-25 Published:2009-12-28

被引次数

9

摘要： 目的建立血液中乙基葡萄糖醛酸苷(ethyl glucuronide,EtG)的气相色谱-串联质谱(GC-MS/MS)测定方法。方法血液用乙腈沉淀蛋白,离心后,将上清液转移吹干,残留物经N,O-双(三甲基硅烷基)三氟乙酰胺衍生化,用GC-MS/MS进行检测。结果血液中EtG的检出限为0.05μg/mL,线性范围为0.1～10μg/mL(r=0.9999)。对送检案例血液检材进行EtG检测,效果良好。结论本方法适用于血液中EtG的检测。

关键词: 法医毒理学, 血液, 乙基葡萄糖醛酸苷, 气相色谱-串联质谱法

Abstract: Objective To develop a method for determining ethyl glucuronide(EtG) in human blood with gas chromatograph-tandem mass spectrometry (GC-MS/MS). Methods Human blood protein was precipitated with acetonitrile. The supernatant was transferred and air flow dried after centrifugated. The residue was derived with N, O -bis (trimethylsilyl)trifluoroacetamide (BSTFA), and analyzed with GC -MS/MS. Results The detection limit of EtG in blood was 0.05 μg/mL. Calibration curve covered a span from 0.1-10 μg/mL with a good linear relationship (r=0.999 9). The method showed a excellent performance when was used to authentic blood sample analysis for EtG. Conclusion The method is suitable for blood EtG analysis.

Key words: forensic toxicology, blood, ethyl glucuronide, GC-MS/MS