法医学杂志

法医学杂志 ›› 2009, Vol. 0 ›› Issue (6): 434-436.

• 论文 • 上一篇    下一篇

保存温度与时间对生物样品中氯胺酮稳定性的影响

曹洁;王振华;贾娟;贠克明;尉志文;王玉瑾;   

  1. 山西医科大学法医学院;西安市公安局刑事侦查局;
  • 发布日期:2009-12-25 出版日期:2009-12-28

Effect of Temperature and Time on Stability of Ketamine in Biological Samples

CAO JIE1, WANG ZHEN-HUA2, JIA JUAN1, YUN KE-MING1, WEI ZHI-WEN1, WANG YU-JIN1 (1. SCHOOL OF FORENSIC MEDICINE, SHANXI MEDICAL UNIVERSITY, TAIYUAN 030001, CHINA; 2. DEPARTMENT OF CRIMINAL INVESTIGATION, PUBLIC SECURITY BUREAU OF XI’AN, XI’AN 710016, CHINA)   

  • Online:2009-12-25 Published:2009-12-28

摘要： 目的研究不同温度和时间条件保存时生物样品中氯胺酮的稳定性。方法家兔以氯胺酮150mg/kg灌胃,30min后处死,取其血、肝、肾、脑,分别在室温(18～24℃)和冷冻(-20℃)条件下保存,并用气相色谱-质谱法定性分析、气相色谱-氮磷检测器法测定不同时间各样品中氯胺酮含量。结果血、肝、肾、脑冷冻保存至第30天氯胺酮含量均降低(P<0.05);室温条件下各样品中氯胺酮含量自第5天起均升高(P<0.05)。结论生物样品在冷冻条件下保存时氯胺酮稳定性较好,怀疑氯胺酮中毒或死亡的检材应冷冻保存,尽快检测。

关键词: 法医毒理学, 氯胺酮, 稳定性, 兔

Abstract: Objective The stability of ketamine in biological samples was studied under different storage temperature and time. Methods The rabbits were given intragastric administration of ketamine with a dose of 150 mg/kg and were sacrificed after 30 minutes. Blood, liver, kidney and brain of the rabbits were stored at room temperature (between 18 ℃ and 24 ℃) and -20 ℃. The specimens were analyzed at different times by GC-MS and GC-NPD. Results At -20 ℃, the concentration of ketamine decreased in all of samples (P<0.05) within 30 days. The concentration of ketamine increased in all of samples stored at room temperature after 5 days(P<0.05). Conclusion The stability of ketamine in biological samples stored at-20 ℃ was better than that at room temperature. The samples suspected containing ketamine should be stored at-20 ℃ and should be tested as soon as possible.

Key words: forensic toxicology, ketamine, stability, rabbits