Abstract: Objective To build the four STR loci typing system by fluorescence labeled Multiplex-PCR technique, applied in the parentage test and personal identification in forensic medicine. Methods The primer of D3S1754 and D1S549 were labeled with 6-FAM and TMR respectively, primers of D4S2366 and D12S375 were labeled with HEX. Multiplex-PCR products were analysed on the ABI PRISMTM 310 Genetic Analyzer where the Data Collection Software 3.0, the GeneScan Analysis Software 3.7NT and the Genotyper?誖3.7NT Software were used. This typing system has been emploied in the parentage test and personal identification of casework. Results A method of typing four STR loci by fluorescence labeled Multiplex-PCR technique had been constructed . It has showed good sensitive and stability, and met the needs of parentage test and personal identification in forensic medicine. Conclusion The constructed method can be used in studying genetic polymorphisms and parentage test or personal identification in forensic medicine.

Key words: multiplex polymerase chain reaction, short tandem repeats, typing, labeled with fluorescence