Abstract: Objective To establish a fluorescent multiple amplification system of 16SrRNA and Cytb genes located in mitochondrial DNA for species identification.Methods A pair of primers of 16SrRNA gene and Cytb gene of the mitochondrial DNA was designed with the software Primer 5.0 to construct a multiple amplification system.The amplified products from human and five species of animals,including cattle,pig,dog,chicken and grass carp were analyzed by 310 Genetic Analyzer.Results The amplified products of these samples showed two peaks.The common one was 358 bp and the specific one different in unique species was between 231 bp and 256 bp.Conclusion The multiplex amplification system can exactly distinguish the species of human from five common animals.

Key words: forensic genetics, biological assay, gene amplification, DNA, mitochordria, genes, 16SrRNA, genes, cytochromes b