Study of Papaver Somniferum Cultivars Identification by TD-RAPD Technique

Abstract

Abstract: Objective The feasibility of Papaver somniferum L. cultivars identification was explored by TD-RAPD technique. Method Genomic DNA was extracted by improved CTAB method. One sample of species from Papaver somniferum L in xishuangbanna area. was studied by using TD-RAPD method. Result We established an optimal method of extracting genomic DNA. Six primers were picked out from 10 primers. Conclusion TD-RAPD could be applied to researches of molecular marker of Papaver somniferum L. TD-RAPD technique provide a method to constitute DNA database of Papaver somniferum L. and conclude the source of opium poppy.

Key words: Papaver somniferum L., DNA, TD-RAPD

LU FAN;HONG JUN-YAN;HE RUI;LI LI-SHA (. YUNNAN CRIMINAL SCIENCE AND TECHNOLOGY INSTITUTE;KUNMING 00;CHINA;. KM BIOLINK CO.;LTD;KUNMING 0;CHINA;. XISHUANGBANNA REGION CRIMINAL SCIENCE AND TECHNOLOGY INSTITUTE;JINGHONG 00;CHINA). Study of Papaver Somniferum Cultivars Identification by TD-RAPD Technique[J]. , 2006, 0(5): 367-369.

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Study of Papaver Somniferum Cultivars Identification by TD-RAPD Technique

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