Abstract: Objective To establish a method for DNA examination on mouth mucosa exfoliative cells from toothbrush bristles. Methods Slough-off mouth mucosa exfoliative cells were collected by bristles extracted method and direct washing method. The DNA of exfoliative cells was extracted with Chelex-100 method and DNA IQTM kit. Then all the extracted DNA took PCR amplification and STR analysis. Results There was a significant difference between the groups with bristles extracted method and with direct washing method at the success rates of over 9 STR loci detected (P<0.05). However, there was no statistical significance between Chelex-100 method and DNA IQTM kit (P>0.05). Conclusion It is demonstrated that mouth mucosa exfoliative cells collected by bristles extracted method is more available than that with direct washing method.

Key words: forensic genetics, extraction, mouth mucosa, exfoliative cell, toothbrush-bristles