法医学杂志

法医学杂志 ›› 1998, Vol. 0 ›› Issue (4): 201-202+.

• 论文 • 上一篇    下一篇

尿中氯喹定性定量分析方法的研究

吴何坚   

  1. 司法部司法鉴定科学技术研究所!上海,200063
  • 发布日期:1998-08-25 出版日期:1998-08-28

Determination of chloroquine in human urine

WU HEJIAN (INSTITUTE OF FORENSIC SCIENCES, MINISTRY OF JUSTICE, PR. CHINA, SHANGHAI 200063 )   

  • Online:1998-08-25 Published:1998-08-28

摘要： 建立了人尿中氯喹的定性定量分析方法，2ml尿样用2ml×2环己烷：乙酸乙酯（8：2）提取净化后，60℃水浴室气吹干，残留物定容溶解后，气相色谱分析，氯喹的保留时间为9．44min。方法最低检测限为200ng／ml，回收率为87．0％，RSD＝7．9％（n＝5），在0～50μg／ml浓度范围内，有良好的线性关系：A＝1778．9＋13686C，r＝0．999。方法同时可用于血中氯喹的分析。附一例应用报告，测得尿中氯喹的含量为0．745mg／ml，血中氯喹的含量为3．68μg／ml。尿液中同时检出氯喹的N－去单已基代谢物。定性结果经质谱法验证。

关键词: 氯喹, 尿液, 气相色谱法

Abstract: A GC method was established for qualitative and quantitative determination of chloroquine in human urine. The chloroquine is extracted from 2ml urine with 2 x 2ml cyclohexame: chloroform (8: 2). The retention time of chloquine is9.44min. The linear of quantitation is 0 - 50μg /ml. Therecovery of the procedure in 25μg g/ml is 87 .0%, CV = 7.9% (n = 5). The limit of the determination is 200ng/ml.The method can also be used in blood. A case is reportedhere. In that, we detected chloroquine in urine and blood.The quantitation of chloroquine in urine and blood is 0. 475mg/ml and 3 .68μg/ml, respectively. A metabolite of chloroquine - desethylchloroquine is also found in the urine.

Key words: chloroquine, urine, GC