法医学杂志

法医学杂志 ›› 2005, Vol. 21 ›› Issue (4): 244-245,248.

• 论文 • 上一篇    下一篇

大鼠弥漫性脑损伤后脑组织HIF-1α的变化规律

苏莉,朱旭阳,汪静宇,张纯兵,陈仁辉,李永宏   

  1. 皖南医学院法医学系,皖南医学院法医学系,皖南医学院法医学系,皖南医学院法医学系,皖南医学院法医学系,皖南医学院法医学系 安徽芜湖241001 ,安徽芜湖241001 ,安徽芜湖241001 ,安徽芜湖241001 ,安徽芜湖241001 ,安徽芜湖241001
  • 发布日期:2005-08-25 出版日期:2005-08-28

Induction of Hypoxia-inducible Factor-1(HIF-1) Following Diffuse Rats Brain Injury

SU LI, ZHU XU-YANG, WANG JING-YU, ET AL. (DEPARTMENT OF FORENSIC MEDICINE, WANNAN MEDICAL COLLEGE, WUHU 241001, CHINA)   

  • Online:2005-08-25 Published:2005-08-28

摘要： 目的探讨大鼠弥漫性脑损伤后脑组织内缺氧诱导因子-1α(HIF-1α)的表达规律及其意义。方法应用免疫组织化学方法和图像分析技术,对大鼠弥漫性脑损伤后不同损伤时间大脑皮层、丘脑和脑干部位神经元细胞内HIF-1α的变化进行研究。结果不同损伤时间,HIF-1α的表达强弱不同,损伤后1～2h可在皮质、丘脑和脑干等部位观察到HIF-1α增多,12h达高峰,24h减弱。结论HIF-1α可作为检测早期脑损伤的生物学指标。

关键词: 缺氧诱导因子-1&alpha, (HIF-1&alpha, ), 免疫组织化学, 脑损伤

Abstract: Objective To investigate the expression of hypoxia-inducible factor-1α(HIF-1α) in neurons after diffuse rats brain injury. Methods On the basis of the Mamarou diffuse brain injury model, the rat was killed at different time after diffuse brain injury, then changes of HIF-1α in neurons of cortex, thalamus and pontine were observed by immunohistochemistry and image analysis techniques. Results The quantities of HIF-1α raised in a fluctuating way after brain injury, and its changes were different in different brain areas. Conclusion HIF-1α is a valuable biomarker for diagnosis of diffuse brain injury.

Key words: hypoxia-inducible factor-1&alpha, (HIF-1&alpha, ), immunohistochemistry, diffuse brain injury