法医学杂志

法医学杂志 ›› 2007, Vol. 0 ›› Issue (2): 84-85+8.

• 论文 • 上一篇    下一篇

大鼠死后肝脏磷酸果糖激酶-2含量的变化

金菊;李斌;焦白光;付佳;黄红;宋宇;彭其毅;廖志钢;   

  1. 四川大学华西基础医学与法医学院,四川华大司法鉴定所,电子科技大学医院,四川大学华西公共卫生学院,四川大学华西基础医学与法医学院,四川大学华西基础医学与法医学院,四川大学华西基础医学与法医学院,四川大学华西基础医学与法医学院 四川成都610041,四川成都610041,四川成都610054,四川成都610041,四川成都610041,四川成都610041,四川成都610041,四川成都610041
  • 发布日期:2007-04-25 出版日期:2007-04-28

Postmortem Changes of Liver Phosphofructokinase-2 Level in Rats Following Different Causes of Death

JIN JU1,LI BIN2,JIAO BAI-GUANG3,FU JIA4,HUANG HONG1,SONG YU1,PENG QI-YI1,LIAO ZHI-GANG1(1.WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE,SICHUAN UNIVERSITY,CHENGDU 610041,CHINA;2.SICHUAN HUADA JUDICAL EXPERT INSTITUTE,CHENGDU 61004,CHINA;3.HOSPITA   

  • Online:2007-04-25 Published:2007-04-28

摘要： 目的分析不同死亡时间和死亡原因大鼠肝脏组织内磷酸果糖激酶-2(PFK-2)含量的变化。方法大鼠105只随机分为三组,以不同方式(失血、窒息、断颈)处死。分别于死后0、1、2、4、8、12和24h取大鼠肝脏组织,免疫组化和图像分析技术测定大鼠PFK-2变化。结果三组不同死亡原因大鼠肝脏组织内PFK-2的含量随死亡时间延长呈规律性减弱趋势。结论大鼠肝脏组织内PFK-2的变化可以反映死亡时间的变化趋势。

关键词: 死亡时间, 磷酸果糖激酶-2, 含量, 肝组织

Abstract: Objective To study the changes of liver phosphofructokinase-2(PFK-2) level at different postmortem intervals as well as due to different causes of death.Methods One hundred and five rats were randomly divided into 3 groups and the rats were sacrificed by either bleeding,suffocating,and neck breaking,respectively.The content of liver PFK-2 at 0,1,2,4,8,12 and 24 hours following death were studied using immunohistochemishtry and image analysis.Results PFK-2 content of the rat's liver in all 3 groups showed a linear decrease at different postmortem intervals with no significant statistical differences found between the different groups.Conclusion PFK-2 level in rat liver appears to decrease linearly in correlation with prolonged PMI.

Key words: postmortem interval, phosphofructokinase-2(PFK-2), content, liver tissue