The Role of Iron Dysregulation in White Matter Injury Induced by Carbon Mono- xide Poisoning

doi:10.12116/j.issn.1004-5619.2023.431104

Abstract

Abstract:

Objective To explore the possible mechanisms of changes in neural metabolic cells in the white matter injury sites of the brain in mice with carbon monoxide （CO） poisoning and the abnormal absorption of iron ions in the body caused by intestinal flora and intestinal flora disorders. Methods C57BL/6 mice were placed in a closed jar and given CO to establish a mouse model of CO poisoning. The control group was given the same treatment but not given CO. Samples were taken from the white matter to observe the morphological changes of white matter, the expression of transferrin （TRF） and glutathione peroxidase 4 （GPX4） was analyzed by immunohistochemistry and Western blotting. Meanwhile, feces of mice were collected at 3rd and 7th day after CO exposure, and 16S rRNA sequencing was performed to analyze the changes of intestinal flora in mice caused by CO poisoning. Results Electron microscopy showed that CO poisoning caused obvious demyelination, with increased expression of TRF and decreased expression of GPX4 in the white matter （P<0.05）. CO poisoning led to abnormal distribution of intestinal flora in mice, resulting in abnormal absorption of iron in intestinal tissue. Conclusion CO poisoning can cause damage to white matter nerve cells in the brain, leading to demyelination. It can also cause abnormal intestinal flora distribution and iron absorption in mice.

Key words: forensic pathology, forensic toxicology, carbon monoxide poisoning, intestinal flora, white matter, demyelination, ferroptosis, mice

CLC Number:

Jin-xuan YANG, Dong-an TIAN, Xin-yu TANG, Zhan-ying ZHAO, Xi-yue JING, Dong-mei WANG, Xiao-fei TIAN. The Role of Iron Dysregulation in White Matter Injury Induced by Carbon Mono- xide Poisoning[J]. Journal of Forensic Medicine, 2025, 41(6): 545-551.

Figures/Tables 6

Fig. 1 Changes in the abundance of intestinal flora in mice caused by CO poisoning

Fig. 2 Structural changes of myelin sheath in the white matter of mice caused by CO poisoning （×8 000）

Tab. 1 Expression changes of TRF and GPX4 in the white matter of mice caused by CO poisoning

组别	TRF		GPX4
组别	平均阳性强度	相对表达量	平均阳性强度	相对表达量
正常对照	6.078±0.283	0.722±0.071	10.766±0.571	0.786±0.070
染毒3 d	19.023±0.222^1）	0.921±0.072^1）	9.031±0.403^1）	0.659±0.083^1）
染毒7 d	8.691±0.243	0.751±0.210	9.119±0.469^1）	0.683±0.087^1）

Fig. 3 Expression changes of TRF in the white matter of mice caused byCO poisoning detected by immunohistochemical staining

Fig. 4 Expression changes of GPX4 in the white matter of mice caused byCO poisoning detected by immunohistochemical staining

Fig. 5 Expression changes of TRF and GPX4 in thewhite matter of mice caused by CO poisoningdetected by Western blotting

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