A Histochemical Quantitative Study on Nuclear DNA in Rat Liver Cell after Death

Abstract

Abstract: Postmortem interval (PMI) estimation is one of the most important problems in forensic autopsy. A microspoetrophotometeric study was conducted on the mean nuclear DNA content in rat liver cell after different time postmortem under different environmental temperature (10℃, 21℃, 30℃), We found rat nuclear DNA content was linear related with PMI under 10℃, 20℃ and 30℃ with correlations of-0. 9905,-0. 9863 and -0. 9702 respectively,and the decrease of nuclear DNA content accelerated under higher environmental temperature. We believe the histochemical quantitatve technique should be a new way to correct estimation of PMI.

Key words: microspectrophotometer, DNA, hepatic cell nuclear, postmortem interval

TI BIN;KONG XIANGLIN;YAN ZHUKANG;GU YUNJU (FACULTY OF FORENSIC MEDICINE;SHANGHAI MEDICAL UNIVERSITY;SHANGHAI 000);CHEN ZHIQIANG;KANG JIANNAN (CENTRAL LABORATORY;SHANGHAI RAILWAY MEDICAL COLLEGE;SHANGHAI 0000). A Histochemical Quantitative Study on Nuclear DNA in Rat Liver Cell after Death[J]. , 1994, 10(4): 0-0.

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A Histochemical Quantitative Study on Nuclear DNA in Rat Liver Cell after Death

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