Abstract: Objective To validate the genotype of 12 STR loci by sequencing method. Methods According to the special sequence of 12 STR loci(CSF1PO, FGA, TH01, TPOX, VWA, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51 and D21S11), the relative PCR primers were designed. PCR products of control DNA(9947A) and STR mutation samples were sequenced. Results The sequencing results of both the control DNA(9947A) and STR mutation samples were consistent with their genotyping results. Conclusion The sequencing method developed are accurate and sensitive, can be used for the validation of STR genotyping results.

Key words: forensic genetics, short tandem repeat, mutation