Abstract: Objective To develop a rapid,accurate and economical real time fluorescence PCR method with TaqMan probe technology to detect the X chromosome single nucleotide polymorphism(X-SNP).Methods Taq-Man probes and polymerase chain reaction primers were respectively designed according to the 13 X-SNP.Then,the X-SNP were genotyped after the amplification by real time fluorescence PCR.Results All the loci follow the Hardy-Weinberg equilibrium.The polymorphic information content for 13 distinct loci varied between 0.349 7 and 0.375 0 while the heterozygosity ranged from 0.453 7 to 0.502 1.A real time fluorescent PCR method based on TaqMan probe was successfully developed and the results were accordant with those analyzed by DNA sequencing of the 13 X-SNP.Conclusion The allele specific real time fluores-cence PCR based on TaqMan probe is a sensitive,simple technology and suitable for rapid analysis of X-SNP.All the loci show highly polymorphic and may be potential in forensic genetics.

Key words: forensic genetics, polymorphism, single nucleotide, X chromosome, TaqMan probe