Journal of Forensic Medicine

›› 2015, Vol. 31 ›› Issue (5): 373-376.DOI: 10.3969/j.issn.1004-5619.2015.05.011

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Development of a Forensic Multiplex Amplification STR Kit for 15 Autosomal STR Loci and 10 Y-STR Loci

DONG YAN1,2, LIN SHUANG-SHUANG3, CAO YU1, WU WEI-WEI4, HUANG SHU-QIN5, ZHENG WEI-GUO6, LI FA-YUAN6, GE BIN-WEN6, GUO YU-LIN6, ZHOU HUAI-GU1   

  1. （1. Shanghai Key Laboratory of Crime Scene Evidence, Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China; 2. School of Life Sciences, Fudan University, Shanghai 200433, China; 3. Changning Branch of Shanghai Municipal Public Security Bureau, Shanghai 200335, China; 4. Department of Criminal Investigation, Zhejiang Provincial Public Security Bureau, Hangzhou 310009, China; 5. Zhengzhou Public Security Bureau, Zhengzhou 450000, China; 6. AGCU ScienTech Incorporation, Wuxi 214174, China）
  • Online:2015-10-25 Published:2015-10-28

Abstract: Objective To establish a multiplex STR genotyping method for autosomal STR and Y-STR loci in forensic biological practice. Methods Widely used autosomal STR loci and Y-STR loci were selected. A set of PCR primers was designed, and a 5-dye fluorescent labeled STR multiplex PCR reagent kit was developed. Results A kit was developed which can simultaneously detect 15 autosomal STR loci, 10 Y-STR loci, and an Amelogenin. Conclusion The 15 autosomal STR plus 10 Y-STR kit in combination with capillary electrophoresis method was used to STR genotyping with accurate and reliable results. The new one-step testing kit can potentially be widely used in forensic cases and DNA databank in the future.

Key words: forensic genetics, reagent kits, short tandem repeat sequences, autosome, Y chromosome

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